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polyclonal rabbit anti-cd63 antibody  (System Biosciences Inc)

 
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    System Biosciences Inc polyclonal rabbit anti-cd63 antibody
    Polyclonal Rabbit Anti Cd63 Antibody, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti-cd63+antibody/anti+cd63/pmc12177847-64-4-19
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-cd63 antibody - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Utilizing Two Electrokinetic Techniques on a Single Device for Detection of Extracellular Vesicle‐Associated Protease Activity
    Article Snippet: Rabbit monoclonal anti‐calnexin antibodies were purchased from Abcam (Cambridge, UK; ab133615, clone# EPR3633(2), lot#GR3416744‐14), and goat anti‐rabbit HRP antibodies were purchased from Cell Signaling Technology (Danvers, MA; 7074S, lot#27). .. Polyclonal rabbit anti‐CD63 and anti‐CD9 antibodies with goat anti‐rabbit HRP secondary antibodies for western blot analysis were purchased from System Biosciences Innovation (Palo Alto, CA; EXOS‐CD63A‐1, lot#190523‐001; EXOS‐CD9A‐1, lot#190911‐002). .. On‐chip immunofluorescent staining utilized monoclonal mouse anti‐CD63 antibodies purchased from Abcam (Cambridge, UK; ab8219, lot#GR3196070‐11) and secondary antibodies AlexaFluor goat anti‐mouse IgG purchased from Invitrogen (Waltham, MA; A21235, lot#2482945).

    other:

    Article Title: Dose-related shifts in proteome and function of extracellular vesicles secreted by fetal neural stem cells following chronic alcohol exposure
    Article Snippet: For immuno-gold labeling of the EV surface marker CD63, polyclonal rabbit anti-CD63 antibody diluted 1:100 (System Biosciences; Catalog # EXOAB-CD63A-1; Palo Alto, CA/USA) was used as the primary antibody, and binding detected incubation with 12 nm goat anti-rabbit antibody conjugated with colloidal gold (Jackson ImmunoResearch Lab; Catalog # 111-205-144; West Grove, PA/USA).



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    Fig. 3. CHKA mediates exosomes secretion in glioma cells. A-C Western blot (WB) analysis of exosome marker proteins <t>CD63</t> and TSG101 in exosomes isolated from the supernatant of glioma cells in shCHKA and shNC group along with the negative control protein Calnexin. D Exosome protein concentration in the shCHKA and shNC groups of glioma cells using the BCA assay. E Electron microscopy was performed on shCHKA and shNC cells in glioma cells (Scale bars = 1 μm). F The number of multivesicular bodies (MVBs) per glioma cell profile. G The number of intraluminal vesicles (ILVs) per MVB profile in glioma cells. *P < 0.05, **P < 0.01, ***P < 0.001. shCHKA: CHKA knockdown group, shNC: con trol group.
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    Fig. 3. CHKA mediates exosomes secretion in glioma cells. A-C Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in shCHKA and shNC group along with the negative control protein Calnexin. D Exosome protein concentration in the shCHKA and shNC groups of glioma cells using the BCA assay. E Electron microscopy was performed on shCHKA and shNC cells in glioma cells (Scale bars = 1 μm). F The number of multivesicular bodies (MVBs) per glioma cell profile. G The number of intraluminal vesicles (ILVs) per MVB profile in glioma cells. *P < 0.05, **P < 0.01, ***P < 0.001. shCHKA: CHKA knockdown group, shNC: con trol group.

    Journal: Biochemical and biophysical research communications

    Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

    doi: 10.1016/j.bbrc.2024.151269

    Figure Lengend Snippet: Fig. 3. CHKA mediates exosomes secretion in glioma cells. A-C Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in shCHKA and shNC group along with the negative control protein Calnexin. D Exosome protein concentration in the shCHKA and shNC groups of glioma cells using the BCA assay. E Electron microscopy was performed on shCHKA and shNC cells in glioma cells (Scale bars = 1 μm). F The number of multivesicular bodies (MVBs) per glioma cell profile. G The number of intraluminal vesicles (ILVs) per MVB profile in glioma cells. *P < 0.05, **P < 0.01, ***P < 0.001. shCHKA: CHKA knockdown group, shNC: con trol group.

    Article Snippet: Antibodies used in the Western blotting process included: rabbit antihuman CHKA polyclonal antibody (abcam, USA), rabbit anti-human GAPDH polyclonal antibody (Affinity, China), rabbit anti-human CD63 polyclonal antibody (Proteintech, China), rabbit anti-human TSG101 polyclonal antibody(Proteintech, China), rabbit anti-human calnexin polyclonal antibody(Proteintech, China), rabbit anti-human LC3B polyclonal antibody(Sigma, USA), and rabbit anti-human P62/SQSTM1 polyclonal antibody(Sigma, USA).

    Techniques: Western Blot, Marker, Isolation, Negative Control, Protein Concentration, BIA-KA, Electron Microscopy, Knockdown

    Fig. 4. CHKA knockdown Inhibited Proliferation, Migration and invasion of glioma Cells by Blocking Exosome Secretion. A Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in DMSO and GW4869 group. (BC) The proliferation of glioma cells U87MG and U251 in DMSO and GW4869 group, the migration ability of glioma cells U87MG (DE) and U251 (DF) in DMSO and GW4869 group (Scale bars = 200 μm). GH The invasion ability of glioma cells U87MG and U251 in DMSO and GW4869 group(Scale bars = 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Biochemical and biophysical research communications

    Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

    doi: 10.1016/j.bbrc.2024.151269

    Figure Lengend Snippet: Fig. 4. CHKA knockdown Inhibited Proliferation, Migration and invasion of glioma Cells by Blocking Exosome Secretion. A Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in DMSO and GW4869 group. (BC) The proliferation of glioma cells U87MG and U251 in DMSO and GW4869 group, the migration ability of glioma cells U87MG (DE) and U251 (DF) in DMSO and GW4869 group (Scale bars = 200 μm). GH The invasion ability of glioma cells U87MG and U251 in DMSO and GW4869 group(Scale bars = 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Antibodies used in the Western blotting process included: rabbit antihuman CHKA polyclonal antibody (abcam, USA), rabbit anti-human GAPDH polyclonal antibody (Affinity, China), rabbit anti-human CD63 polyclonal antibody (Proteintech, China), rabbit anti-human TSG101 polyclonal antibody(Proteintech, China), rabbit anti-human calnexin polyclonal antibody(Proteintech, China), rabbit anti-human LC3B polyclonal antibody(Sigma, USA), and rabbit anti-human P62/SQSTM1 polyclonal antibody(Sigma, USA).

    Techniques: Knockdown, Migration, Blocking Assay, Western Blot, Marker, Isolation

    Fig. 6. CHKA attenuates exosomes release through Autophagy-Mediated mechanism AB Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U87MG cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. AC Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U251 cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. D Exosome protein concentration in the s shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 groups of glioma cells using the BCA assay. **P < 0.01, ***P < 0.001. Baf-A1: Bafilomycin A1.

    Journal: Biochemical and biophysical research communications

    Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.

    doi: 10.1016/j.bbrc.2024.151269

    Figure Lengend Snippet: Fig. 6. CHKA attenuates exosomes release through Autophagy-Mediated mechanism AB Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U87MG cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. AC Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of U251 cells in shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 group. D Exosome protein concentration in the s shNC + DMSO, shCHKA + DMSO and shCHKA + Baf-A1 groups of glioma cells using the BCA assay. **P < 0.01, ***P < 0.001. Baf-A1: Bafilomycin A1.

    Article Snippet: Antibodies used in the Western blotting process included: rabbit antihuman CHKA polyclonal antibody (abcam, USA), rabbit anti-human GAPDH polyclonal antibody (Affinity, China), rabbit anti-human CD63 polyclonal antibody (Proteintech, China), rabbit anti-human TSG101 polyclonal antibody(Proteintech, China), rabbit anti-human calnexin polyclonal antibody(Proteintech, China), rabbit anti-human LC3B polyclonal antibody(Sigma, USA), and rabbit anti-human P62/SQSTM1 polyclonal antibody(Sigma, USA).

    Techniques: Western Blot, Marker, Isolation, Protein Concentration, BIA-KA